Journal:

Article Title: Circulating immune complexes in IgA nephropathy consist of IgA1 with galactose-deficient hinge region and antiglycan antibodies

doi:

Figure Lengend Snippet: Binding of IgG from normal human sera to IgA1 and IgA2 myeloma proteins and Fab IgA1 with intact and modified hinge region glycans

Article Snippet: Biotinylated polyclonal antibodies to IgM and F(ab′) 2 fragment of anti-IgA and anti-IgG (all heavy chain specific) were purchased from Southern Biotechnology Associates Inc. (Birmingham, Alabama, USA).

Techniques: Binding Assay, Modification

Journal:

Article Title: Circulating immune complexes in IgA nephropathy consist of IgA1 with galactose-deficient hinge region and antiglycan antibodies

doi:

Figure Lengend Snippet: Correlation of binding of HAA and normal serum IgG to IgA myeloma proteins. The binding of serum IgG to IgA1 (filled squares) and IgA2 (open circle) myeloma proteins. The binding of IgG to IgA1 significantly correlated with HAA binding (r = 0.875, P = 0.044), indicating requirement of terminal GalNAc residues for IgG binding.

Article Snippet: Biotinylated polyclonal antibodies to IgM and F(ab′) 2 fragment of anti-IgA and anti-IgG (all heavy chain specific) were purchased from Southern Biotechnology Associates Inc. (Birmingham, Alabama, USA).

Techniques: Binding Assay

Journal:

Article Title: Circulating immune complexes in IgA nephropathy consist of IgA1 with galactose-deficient hinge region and antiglycan antibodies

doi:

Figure Lengend Snippet: Binding of serum of IgG from IgAN patients and healthy controls to intact and modified IgA myeloma proteins and their Fab fragments

Article Snippet: Biotinylated polyclonal antibodies to IgM and F(ab′) 2 fragment of anti-IgA and anti-IgG (all heavy chain specific) were purchased from Southern Biotechnology Associates Inc. (Birmingham, Alabama, USA).

Techniques: Binding Assay, Modification

Journal:

Article Title: Circulating immune complexes in IgA nephropathy consist of IgA1 with galactose-deficient hinge region and antiglycan antibodies

doi:

Figure Lengend Snippet: The binding of serum IgG to Fab fragment of IgA1 (Ste) myeloma protein. Wells of microtiter plates were coated with Fab fragment of IgA1, incubated with diluted sera from 20 IgAN patients, 20 healthy controls, and 20 patients with non-IgA GN and subsequently with biotinylated mAb specific for IgG, avidin-alkaline phosphatase, and phosphatase substrate. Data shown are OD at 405 nm, mean and SD. Statistical significance is noted; NS, not significant.

Article Snippet: Biotinylated polyclonal antibodies to IgM and F(ab′) 2 fragment of anti-IgA and anti-IgG (all heavy chain specific) were purchased from Southern Biotechnology Associates Inc. (Birmingham, Alabama, USA).

Techniques: Binding Assay, Incubation, Avidin-Biotin Assay

Journal:

Article Title: Circulating immune complexes in IgA nephropathy consist of IgA1 with galactose-deficient hinge region and antiglycan antibodies

doi:

Figure Lengend Snippet: Subclass specificity of serum IgG binding to a,a-IgA1. Wells of microtiter plates were coated with myeloma a,a-IgA1, incubated with sera (diluted 1:10) from 30 IgAN patients and 30 healthy controls (C) and subsequently with biotinylated mAb specific for IgG1, IgG2, IgG3, and IgG4; avidin-alkaline phosphatase; and phosphatase substrate. Data shown are OD at 405 nm, mean and SD.

Article Snippet: Biotinylated polyclonal antibodies to IgM and F(ab′) 2 fragment of anti-IgA and anti-IgG (all heavy chain specific) were purchased from Southern Biotechnology Associates Inc. (Birmingham, Alabama, USA).

Techniques: Binding Assay, Incubation, Avidin-Biotin Assay

Journal:

Article Title: Circulating immune complexes in IgA nephropathy consist of IgA1 with galactose-deficient hinge region and antiglycan antibodies

doi:

Figure Lengend Snippet: Distribution of IgM, IgA, IgG, and reactivity with HAA in serum fractions from Superose 6 column. Serum from an IgAN patient was fractionated on a Superose 6 column (0.9 × 58.5 cm); 0.25-mL fractions were collected and analyzed by ELISA for the content of IgM (squares), IgA (circles), IgG (open triangles), and reactivity with HAA (filled triangles). Fractions containing aberrantly glycosylated IgA were pooled (HAA pool) and used in the inhibition experiment.

Article Snippet: Biotinylated polyclonal antibodies to IgM and F(ab′) 2 fragment of anti-IgA and anti-IgG (all heavy chain specific) were purchased from Southern Biotechnology Associates Inc. (Birmingham, Alabama, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Inhibition

Journal:

Article Title: Circulating immune complexes in IgA nephropathy consist of IgA1 with galactose-deficient hinge region and antiglycan antibodies

doi:

Figure Lengend Snippet: Inhibition of IgA1-IgG complex formation. IgA1-IgG CICs from an IgAN patient (left columns 1–6) and a healthy control (right columns 1–6) prepared by size-exclusion chromatography on Superose 6 were dissociated at pH 3.0. Aliquots of dissociated CICs were incubated with agarose- or Sepharose-immobilized inhibitors: 1, IgA1(Mce); 2, a,a-IgA1(Mce); 3, GalNAc; 4, GlcNAc; 5, a-OSM; and 6, HSA. The mixture was adjusted to neutral pH to allow re-formation of immune complexes, and the affinity of IgG toward the particular inhibitor was determined as described in Methods.

Article Snippet: Biotinylated polyclonal antibodies to IgM and F(ab′) 2 fragment of anti-IgA and anti-IgG (all heavy chain specific) were purchased from Southern Biotechnology Associates Inc. (Birmingham, Alabama, USA).

Techniques: Inhibition, Size-exclusion Chromatography, Incubation

Journal:

Article Title: Circulating immune complexes in IgA nephropathy consist of IgA1 with galactose-deficient hinge region and antiglycan antibodies

doi:

Figure Lengend Snippet: Binding of serum IgA1, IgM, and IgG from IgAN patients and healthy controls to Fab fragment of IgA1 (Ste) and partially de-O-glycosylated Fab fragment of IgA1 (Ste)

Article Snippet: Biotinylated polyclonal antibodies to IgM and F(ab′) 2 fragment of anti-IgA and anti-IgG (all heavy chain specific) were purchased from Southern Biotechnology Associates Inc. (Birmingham, Alabama, USA).

Techniques: Binding Assay

Journal: iScience

Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers

doi: 10.1016/j.isci.2023.106310

Figure Lengend Snippet:

Article Snippet: Alexa Fluor 555 Goat anti-IgM (Polyclonal) , SouthernBiotech , Cat# 1020–32; RRID: AB_2794223.

Techniques: Purification, Recombinant, Virus, Saline, Plasmid Preparation, Microscopy, Reverse Transcription, Software